A comprehensive study of personal and social information use in female brown-headed cowbirds, Molothrus ater

Brood parasites face considerable cognitive challenges when locating and selecting host nests for their young. One aspect of this challenge is determining how to use different sources of information to make decisions regarding the quality of a prospective nest. Here we investigate how female-brown-headed cowbirds, Molothrus ater, use information when prospecting for nests, and then expand upon this to investigate decisions related to foraging. In chapter 1, we demonstrated female could use social information acquired from observing the nest prospecting patterns of conspecifics to influence their own patterns of nest selection. Furthermore, we found a negative relationship between a female’s accuracy at using personal information and her tendency to copy others. In chapter 2, we found the females were able to use social information in a foraging setting as well. The female’s accuracy using personal information remained consistent across nest prospecting and foraging contexts however, the relationship between accuracy and tendency to copy others drastically reversed. A follow up experiment revealed the likely possibility that the differing relationship between personal and social information use depended on the degree of conflict that existed between the two types of information. In chapter 3, we redeveloped and implemented a new RFID tracking technology allowing us to investigate how the cognitive strategies from chapters 1 and 2 translated to a naturalistic, socially complex breeding environment. We found female cowbirds who spent more time prospecting, produced a greater quantity of eggs and demonstrated high accuracy scores during chapter 1 and 2, whereas females who relied on copying others spent significantly less time prospecting and demonstrated lower laying accuracy scores. By demonstrating how individuals’ cognitive strategies relate across context and translate to a socially complex setting, we have demonstrated the importance of examining behaviour in both of these settings and our RFID tracking technology provides researchers with the framework to effectively study this in the future

Completing the hadronic Higgs boson decay at order αs4\alpha_s^4

We compute four-loop corrections to the hadronic decay of the Standard Model Higgs boson which are induced by effective couplings to bottom quarks and gluons, mediated by the top quark. Our numerical results are comparable in size to the purely massless contributions which have been known for a few years. The results presented in this paper complete the order $\alpha_s^4$ corrections to the hadronic Higgs boson decay.Comment: 15 pages, 2 figure

Hadronic Higgs boson decay at order αs4\alpha_s^4 and αs5\alpha_s^5

We compute corrections to the decay of the Standard Model Higgs boson to hadrons, to the fourth order in the strong coupling constant $\alpha_s$. We use an effective theory in which the Higgs boson couples directly to bottom quarks and to gluons, via top quark--mediated effective couplings. Numerically, our results are of a comparable size to the previously-known "massless" contributions and complete the order $\alpha_s^4$ corrections to the hadronic decay of the Higgs boson. In these proceedings we also provide an independent cross check of the gluonic Higgs boson decay at order $\alpha_s^5$.Comment: 6 pages, 0 figures. Contribution to the proceedings of the XXV International Workshop on Deep-Inelastic Scattering and Related Topics (DIS2017), 3-7 April 2017, University of Birmingham, UK. V2: Reference adde

Complexation of norfloxacin with DNA in the presence of caffeine

1H NMR spectroscopy (500 MHz) has been used to quantify the complexation of the antibacterial antibiotic Norfloxacin (NOR) with DNA in the presence of Caffeine (CAF). Separate studies have been made for the self-association of NOR, its hetero-association with CAF and complexation with a model self-complementary DNA tetramer, 5′-d(TpGpCpA), in order to determine the equilibrium parameters (induced chemical shifts, association constants, enthalpy and entropy) of the two-component mixtures to aid the analysis of the three-component systems. Investigations of the self-association of NOR and its hetero-association with CAF show that the aggregation of NOR molecules and association with CAF in solution are driven by the stacking of aromatic chromophores. The complexation of NOR with d(TGCA) has been analysed in terms of intercalation with the double-stranded form and non-intercalative binding with the single-stranded form of DNA. Investigations of the competitive binding of NOR and CAF with DNA show that at physiological concentrations of NOR (μM) and CAF (mM) the dominant mechanism influencing the affinity of NOR with DNA is the displacement of bound NOR molecules from DNA due to CAF–DNA complexation (i.e. the protector action of Caffeine)

Environmental Implications of the Foodservice and Food Retail Industries

The growing size and importance of service sector industries in the U.S. economy raises questions about the suitability of the current environmental management system to deal with perhaps a changing set of environmental concerns. This paper analyzes the environmental impacts associated with the activities undertaken and influenced by two service sector industries—foodservice (e.g., restaurants) and food retail (e.g., grocery stores). This paper is not a definitive analysis of the magnitude of the environmental effects of these industries, but is intended to be a comprehensive survey of the types of environmental implications—positive and negative—of these two service sectors. The foodservice and food retail industries are components of a larger industrial system, the food marketing system, that extends from the production of food to the marketing of food products to consumers. The U.S. foodservice industry comprises an estimated 831,000 individual establishments, employs an estimated 11 million people (about 8.6% of the U.S. workforce), and is expected to have total sales of $376 billion in 2000. The U.S. food retail industry encompasses approximately 126,000 grocery stores, employs approximately 3.5 million people (about 2.7$449 billion in 1998. For this analysis, we use a simple conceptual framework that segregates the environmental impacts of these industries into three categories: direct, upstream, and downstream. We conclude that, while the direct environmental impacts (e.g., energy use, solid waste generation; air and water emissions; food safety concerns; refrigerants) of these industries are important to recognize and address, opportunities also exist for these industries to address their upstream and downstream environmental impacts.

Measurement of substrate thermal resistance using DNA denaturation temperature

Heat Transfer and Thermal Management have become important aspects of the developing field of uTAS systems particularly in the application of the the uTAS philosophy to thermally driven analysis techniques such as PCR. Due to the development of flowing PCR thermocyclers in the field of uTAS, the authors have previously developed a melting curve analysis technique that is compatible with these flowing PCR thermocyclers. In this approach a linear temperature gradient is induced along a sample carrying microchannel. Any flow passing through the microchannel is subject to linear heating. Fluorescent monitoring of DNA in the flow results in the generation of DNA melting curve plots. This works presents an experimental technique where DNA melting curve analysis is used to measure the thermal resistance of microchannel substrates. DNA in solution is tested at a number of different ramp rates and the di®erent apparent denaturation temperatures measured are used to infer the thermal resistance of the microchannel substrates. The apparent variation in denaturation temperature is found to be linearly proportional to flow ramp rate. Providing knowledge of the microchannel diameter and a non-varying cross-section in the direction of heat flux the thermal resistance measurement technique is independent of knowledge of substrate dimensions, contact surface quality and substrate composition/material properties. In this approach to microchannel DNA melting curve analysis the difference between the measured and actual denaturation temperatures is proportional to the substrate thermal resistance and the ramp-rate seen by the sample. Therefore quantitative knowledge of the substrate thermal resistance is required when using this technique to measure accurately DNA denaturation temperatur

Stochastic models (cooperative and non-cooperative) for NMR analysis of the hetero-association of aromatic molecules in aqueous solution

Stochastic cooperative (STOCH-C) and non-cooperative (STOCH-NC) models have been developed for NMR analysis of the hetero-association of aromatic compounds in solution, in order to take into account all physically meaningful association reactions of molecules in which there are no limitations on the lengths of the aggregates and complexes. These algorithmical approaches are compared with previously published basic (BASE) and generalized (GEN) analytical statistical thermodynamical models of hetero-association of biologically active aromatic molecules using the same sets of published NMR data measured under the same solution conditions (0.1 M phosphate buffer, pD = 7.1, T = 298 K). It is shown that, within experimental errors, the BASE analytical model may be used to describe molecular systems characterized by relatively small contributions of hetero-association reactions, whereas the GEN model may be applied to hetero-association reactions of any aromatic compound with different self-association properties. The STOCH-C computational algorithm enabled the effect on hetero-association of the interactions of molecules with different cooperativity parameters of self-association to be estimated for the first time and it is proposed that the algorithm for the stochastic models has great potential for detailed investigation and understanding of the interactions of aromatic molecules in solution